SubtiBank SubtiBank
yorL [2019-08-05 09:45:56]
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yorL [2019-08-05 09:45:56]

similar to DNA polymerase III (alpha subunit)
locus
BSU_20340
pI
5.94
mw
150.31 kDa
protein length
1305 aa Sequence Blast
gene length
3918 bp Sequence Blast
function
unknown
product
unknown
essential
no
ec
2.7.7.7
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.2|DNA replication/ based on similarity]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]
  • Gene

    Coordinates
    2,175,596 2,179,513

    The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family

  • DNA polymerase type-C family (with [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|PolC] and [protein|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|DnaE], according to UniProt)
  • Structure

  • [PDB|3E0D] (from Thermus aquaticus, corresponds to aa 4 ... 1010 of YorL, 28% identity) [pubmed|18691598]
  • Biological materials

    Mutant

  • BKE20340 ([gene|48BC0FA9901F174472EB070C539EC75EC7C10E88|yorL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE20340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACATCCTATCAACTTTTCTC, downstream forward: _UP4_TAAATCTTAACTGATATAGG
  • BKK20340 ([gene|48BC0FA9901F174472EB070C539EC75EC7C10E88|yorL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK20340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACATCCTATCAACTTTTCTC, downstream forward: _UP4_TAAATCTTAACTGATATAGG
  • References

    Research papers

  • 18691598